Cy3 DBCO

A140-1

1 mg

2580.0

ClickChemistryTools

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A104-5

5 mg

8500.0

ClickChemistryTools

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A104-25

25 mg

23900.0

ClickChemistryTools

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A104-100

100 mg

59000.0

ClickChemistryTools

现询

Abs/Em Maxima: 553/569 nm

Extinction Coefficient: 150,000

Flow Cytometry Laser Line: 532, 555 or 568 nm

Microscopy Laser Line: 532 or 555 nm

Spectrally Similar Dyes: Alexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549

Cy3

Cy3 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye or any other Cyanine3 based fluorescent dyes.

Cy3 DBCO is a bright, far-red-fluorescent, probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. Cy3 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Cy3 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Cy3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine5 based fluorescent dyes.

Cy®3 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

This is sulfonated dye is also known as sulfo-Cyanine3.

分子量

983.18

分子式

N/A

CAS

1782950-79-1

溶(解)度

Water, DMSO, DMF

纯度

N/A

外观

>95% (HPLC)

储存环境

-20°C. Desiccate

运输条件

Ambient temperature

1. Sato, S., et al. (2020). Site-Selective Protein Chemical Modification of Exposed Tyrosine Residues Using Tyrosine Click Reaction. Bioconjugate Chem., 31(5), 1463-73. [PubMed]

2. Allo, B., et al. (2018). Clickable and High-Sensitivity Metal-Containing Tags for Mass Cytometry. Bioconjugate Chem. , 29(6), 2028-38. [PubMed]