Click-&-Go Plus Protein Enrichment Kit*for capture of alkyne-modified proteins*
Click-&-Go® Plus Protein Enrichment Kit is an efficient tool for capture of alkyne-modified proteins on picolyl azide-agarose resin. The alkyne-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the picolyl azide-agarose resin supplied. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. Upon protease digestion, this yields a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis.
Click-&-Go® Plus Protein Enrichment Kit *for capture of alkyne-modified proteins*Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor® 350, CF® 350, DyLight 350, AMCAAZDye™ 350 is a blue-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click react…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405AZDye™ 405 Azide is a water-soluble, pH-insensiti…
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor® 350, CF® 350, DyLight 350, AMCAAZDye™ 350 Alkyne (Alexa Fluor® 350 Alkyne equivalent) is a blue-fluorescent, alkyne-activated probe that reacts with azid…
Abs/Em Maxima: 430/537 nmExtinction Coefficient: 15,000Spectrally Similar Dyes: Alexa Fluor® 430, CF® 430AZDye™ 430 Azide is a water-soluble, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click re…
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor® 350, CF® 350, DyLight 350, AMCAAZDye™ 350 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not re…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405AZDye™ 405 Alkyne reacts with azides via a copper…
Abs/Em Maxima: 494/517 nmExtinction Coefficient: 73.000Flow Cytometry Laser Line: 488 nmMicroscopy Laser Line: 488 nmSpectrally Similar Dyes: Fluorescein, Alexa Fluor® 488, CF® 488A, DyLight® 488, Atto™ 488AZDye™ 488 Azide (Alexa Fluor® 488 Azi…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405ZDye™ 405 DBCO reacts with azides via a copper-fr…
